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. 2005 Nov 25;1(5):e69. doi: 10.1371/journal.pgen.0010069

Figure 2. Respiration Is Not Required for Life Span Extension by CR in PSY316.

Figure 2

(A) PSY316AUT rho0 strains lack mitochondrial DNA. DAPI staining of PSY316 (i) wild-type or (ii) rho0 cells grown under standard conditions (2% glucose) and calorie-restricted (iii) wild-type or (iv) rho0 cells (CR = 0.05% glucose).

(B) PSY316AUT rho0 strains are unable to grow on glycerol as the sole carbon source. (i) PSY316AUT wild-type, (ii) cyt1Δ rho0, (iii) cyt1Δ, or (iv) rho0 cells on YEP supplemented with 2% glucose or 3% glycerol.

(C) CR increases life span in PSY316AUT rho0 cells. Replicative life span analysis for PSY316AUT wild-type and rho0 cells on 2% glucose and 0.05% glucose (CR). Mean life span is shown in parentheses.

(D) CR increases the life span of cyt1Δ cells. Replicative life span analysis for PSY316AUT wild-type and cyt1Δ cells on 2% glucose and 0.05% glucose (CR). Mean life span is shown in parentheses.

(E) CR increases the life span of cyt1Δ rho0 cells. Replicative life span analysis for PSY316AUT wild-type and cyt1Δ rho0 cells on 2% glucose and cyt1Δ rho0 cells on 0.05% glucose (CR). Mean life span is shown in parentheses.