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. 2005 Nov 25;1(5):e69. doi: 10.1371/journal.pgen.0010069

Figure 3. Effect of Glucose Concentration on Life Span and Sir2 Activity in Respiratory-Deficient Mutants.

Figure 3

(A) Mean replicative life span is significantly increased in PSY316 AUT cyt1Δ and rho0 cells as the glucose concentration is decreased to either 0.5% or 0.05%, relative to life span on 2% glucose. *p < 0.05, **p < 0.01.

(B) Sir2 activity is not increased by CR but is responsive to increased expression of Sir2 or to addition of exogenous nicotinamide. Transcriptional silencing of the telomeric URA3 marker in PSY316AUT (WT) was monitored by the survival of cells plated onto medium containing 5-FOA.

(C) Sir2 activity is not altered in respiratory deficient cyt1Δ cells and is unaffected by CR. Transcriptional silencing of the telomeric URA3 marker in PSY316AUT (WT) was monitored by the survival of cells plated onto medium containing 5-FOA.