Fig. 2.

Effects of small RNAs eNOS gene transcription. Nuclei of HAECs were isolated and used in the nuclear run-on transcription reaction containing 2.5 mmol/liter of ATP, CTP, GTP, biotin-16-UPT, and various small RNAs as indicated in the legend. After the reaction at 30°C for 45 min, the biotinylated RNA was separated from total RNA, and used for cDNA synthesis and real-time PCR. The transcription efficiency was presented by the relative mRNA levels of the eNOS and β-actin. Data presented were obtained from three independent experiments; *, P < 0.05; **, P < 0.01 by independent Student's t test when compared to the control.