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Spectral change of ChuS reconstituted with heme, monitored over time after ascorbic acid addition. Absorbance measurements between 500 and 700 nm have been multiplied by 5 to amplify the change in the β- and α-bands. Arrows follow changes in absorption at 408, 545, and 680 nm during reaction progression. The spectral change corresponds with the formation of the product, biliverdin.
