TABLE 2.
Distribution of nucleotide and amino acid substitutions in the PBD of PBP 2X from clinical isolates of S. pneumoniae
| Isolate | Penicillin MIC (μg/ml) | No. (%) ofa:
|
Amino acid motifb
|
|||
|---|---|---|---|---|---|---|
| Nucleotides altered | Amino acids altered | STMK | AHSSNV | LKSGT | ||
| 6190 | 4 | 177 (17.7) | 38 (11.4) | -A-- | ------ | V---- |
| 8111 | 4 | 177 (17.7) | 38 (11.4) | -A-- | ------ | V---- |
| 6363 | 2 | 177 (17.7) | 38 (11.4) | -A-- | ------ | V---- |
| 742 | 2 | 177 (17.7) | 38 (11.4) | -A-- | ------ | V---- |
| 2848 | 2 | 177 (17.7) | 38 (11.4) | -A-- | ------ | V---- |
| 3455 | 1 | 177 (17.7) | 38 (11.4) | -A-- | ------ | V---- |
| 14126 | 0.5 | 177 (17.7) | 38 (11.4) | -A-- | ------ | V---- |
| 11413 | 0.25 | 67 (6.7) | 12 (3.6) | ---- | -L---- | ----- |
| 3996 | 0.12 | 57 (5.7) | 9 (2.7) | -A-- | ------ | ----- |
| 12276 | 0.12 | 57 (5.7) | 9 (2.7) | -A-- | ------ | ----- |
| 3203 | 0.06 | 1 (0.1) | 1 (0.1) | ---- | ------ | ----- |
| 11184 | 0.06 | 1 (0.1) | 1 (0.1) | ---- | ------ | ----- |
| 12244 | 0.06 | 57 (5.7) | 9 (2.7) | -A-- | ------ | ----- |
| 14016 | 0.06 | 3 (0.3) | 0 | ---- | ------ | ----- |
| 8099 | ≤0.03 | 3 (0.3) | 1 (0.3) | ---- | ------ | ----- |
a
Published sequence of penicillin-susceptible S. pneumoniae R6 was used for comparison.
b
Only amino acid residues different from the PBP 2X conserved motif sequences of S. pneumoniae R6 are shown. Conserved amino acid motifs are underlined.