TABLE 4.
Distribution of nucleotide and amino acid substitutions in the PBD of PBP 1A from clinical isolates of S. pneumoniae
| Isolate | Penicillin MIC (μg/ml) | No. (%) ofa:
|
Amino acid motifb
|
|||
|---|---|---|---|---|---|---|
| Nucleotides altered | Amino acids altered | STMK | SRNVP | KTG | ||
| 6190 | 4 | 170 (18.3) | 36 (11.6) | -A-- | ----T | --- |
| 8111 | 4 | 170 (18.3) | 36 (11.6) | -A-- | ----T | --- |
| 6363 | 2 | 170 (18.3) | 36 (11.6) | -A-- | ----T | --- |
| 742 | 2 | 170 (18.3) | 36 (11.6) | -A-- | ----T | --- |
| 2848 | 2 | 170 (18.3) | 36 (11.6) | -A-- | ----T | --- |
| 3455 | 1 | 170 (18.3) | 36 (11.6) | -A-- | ----T | --- |
| 14126 | 0.5 | 203 (21.8) | 43 (13.9) | -S-- | ----T | --- |
| 11413 | 0.25 | 4 (0.4) | 1 (0.1) | ---- | ----- | --- |
| 3996 | 0.12 | 6 (0.6) | 2 (0.6) | ---- | ----- | --- |
| 12276 | 0.12 | 5 (0.5) | 2 (0.6) | ---- | ----- | --- |
| 3203 | 0.06 | 6 (0.6) | 1 (0.1) | ---- | ----- | --- |
| 11184 | 0.06 | 5 (0.5) | 2 (0.6) | ---- | ----- | --- |
| 12244 | 0.06 | 6 (0.6) | 3 (1.0) | ---- | ----- | --- |
| 14016 | 0.06 | 6 (0.6) | 1 (0.1) | ---- | ----- | --- |
| 8099 | ≤0.03 | 4 (0.4) | 2 (0.6) | ---- | ----- | --- |
a
Published sequence of penicillin-susceptible S. pneumoniae R6 was used for comparison.
b
Only amino acid residues different from the PBP 1A conserved motif sequences of S. pneumoniae R6 are shown. Conserved amino acid motifs are underlined.