Fig. 2.
Assays of DNA helical structure with and without thiol linkers present. (a) Melting temperature curves for each of the molecules in the SNP series without a thiol linker attached to the molecule. (b) Melting curves for each of the molecules with thiol linkers attached to each of the 3′ ends. (c) CD measurements on the samples without linkers. (d) The CD curves from the molecules with linkers. Data show that the thiol linkers have no effect upon melting or structure, and that the DNA is in a stable B-form at experimental temperature. —, T:A; - - -, C:G; · · · ·, T:G; - · - · -, C:A.