Fig. 6.

Monte Carlo simulation of the interaction of myosin with unregulated (a and b) and regulated (c and d) actin. These data represent simulations of experimental data similar to that in Fig. 2, which is included as Insets.(a and b) Simulated laser trap displacement data at low surface density (0.1 μg/ml myosin) (a) and high surface density (10 μg/ml myosin) (b) where continuous velocity is observed. (c and d) Simulated data with regulated thin filaments at low surface density (0.1 μg/ml myosin the event shown was the only event in a 20-s record) (c) and high myosin surface density (10 μg/ml myosin) (d). See Appendix for simulation conditions.