Fig. 2. Effects of amino acid residues and position on degron potency.

(A) Overlaid degron score distribution (red) with correlation scatter plot comparing the degron scores of 164 random tiles (blue) with their relative GFP:mCherry ratio as measured by FC and normalized by the GFP:mCherry ratio of the stable tile ENSG00000118898_tile072. Degron score error bars indicate SD of replicates and GFP:mCherry error bars indicate standard error of the mean. (B) Heatmap showing the averaged degron score of tiles with each amino acid at each position. Red indicates a high average degron potency, and blue indicates a low average degron potency. For example, the average degron score of all tiles with an A at position −1 is 0.59 and, hence, appears as red in the heatmap. (C) Bar plot where each bar shows the average score of the 25 first positions from (B) for each amino acid. The cyan line indicates the total average score of all tiles, which is 0.48. (D) Heatmap showing the difference of the average degron score of tiles with exactly one of each amino acid at each position from the average degron score of tiles with exactly one of each amino acid at any other position. Yellow indicates an increase in degron potency at that particular position, and blue indicates a decrease. Black dots indicate statistical significance after Bonferroni correction based on Mann-Whitney U test (P < 0.05/600).