TABLE 1.
Amino acid compositions, molecular masses and proposed structures of muropeptides present in peptidoglycan of S. aureus strain COL grown in the presence of d-serine or d-threonine
| Peaka | Amino acid compositionb
|
Molecular mass [M-H]− | Proposed structurec | |||||
|---|---|---|---|---|---|---|---|---|
| GLX | LYS | ALA | GLY | SER | THR | |||
| Thr1 | 1 | 1.3 | 2.4 | —d | 0.3 | 1.1 | 996.8 | G-M-Ala-Gln-Lys-Ala-Thr |
| Thr3 | 1 | 1.2 | 2.3 | 5.4 | 0.3 | 1.0 | 1,282.1 | G-M-Ala-Gln-Lys (Gly)5-Ala-Thr |
| Thr7 | 1 | 1.2 | 2.3 | 5.6 | 0.3 | 0.5 | 2,446.6 | G-M-Ala-Gln-Lys (Gly)5-Ala-Thr |
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| Thr8 | NDe | ND | ND | ND | ND | ND | 3,609.8 | G-M-Ala-Gln-Lys (Gly)5-Ala-Thr |
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| Thr9 | ND | ND | ND | ND | ND | ND | 4,774.9 | G-M-Ala-Gln-Lys (Gly)5-Ala-Thr |
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| Thr10 | ND | ND | ND | ND | ND | ND | 5,939.3 | G-M-Ala-Gln-Lys (Gly)5-Ala-Thr |
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| Ser1 | 1 | 1.1 | 1.1 | — | 1.9 | — | ND | G-M-Ala-Gln-Lys-Ser-Ser |
| Ser2 | 1 | 1.2 | 2.3 | — | 1.3 | — | 983.1 | G-M-Ala-Gln-Lys-Ala-Ser |
| Ser3 | 1 | 1.2 | 1.1 | 5.4 | 2.2 | — | 1,284.3 | G-M-Ala-Gln-Lys (Gly)5-Ser-Ser |
| Ser4 | 1 | 1.2 | 2.4 | 5.6 | 1.3 | — | 1,268.3 | G-M-Ala-Gln-Lys (Gly)5-Ala-Ser |
| Ser6 | 1 | 1.0 | 1.2 | 5.2 | 1.5 | — | 2,464.7 | G-M-Ala-Gln-Lys (Gly)5-Ser-Ser |
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ser | ||||||||
| Ser7 | 1 | 1.1 | 1.7 | 5.6 | 1.3 | — | 2,448.4 | G-M-Ala-Gln-Lys (Gly)5-Ala-Ser |
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ser | ||||||||
| Ser8 | 1 | 1.1 | 1.7 | 5.5 | 1.3 | — | 2,448.4 | G-M-Ala-Gln-Lys (Gly)5-Ser-Ser |
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| Ser9 | 1 | 1.1 | 2.3 | 5.7 | 0.8 | — | 2,432.2 | G-M-Ala-Gln-Lys (Gly)5-Ala-Ser |
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| Ser10 | ND | ND | ND | ND | ND | ND | 3,612.7 | G-M-Ala-Gln-Lys (Gly)5-Ser-Ser |
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| Ser11 | ND | ND | ND | ND | ND | ND | 3,595.3 | G-M-Ala-Gln-Lys (Gly)5-Ala-Ser |
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
| | | ||||||||
| G-M-Ala-Gln-Lys (Gly)5-Ala | ||||||||
a
See Fig. 1.
b
Values are normalized to that for (glutamine/glutamate) (GLX). LYS, lysine; ALA, alanine; GLY, glycine; THR, threonine; SER, serine.
c
G, N-acetylglucosamine; M, N-acetyl muramic acid.
d
—, less than 0.1.
e
ND, not determined.