Table 3.

Primers for Mutagenesis Constructs^[Note]

	Primer
Mutation	Mutagenesis	Selection (for SV-poly vector)
5′ FLAG	5′-CGCGCCAACACCATGGACTATAAGGACGACGACGACAAGGCGACGGACGCGGCGC3′	5′-CCGCATGCGAATTCGAGCTCTCC-3′
C889T	5′-CCGAAGCCTCTGGTTTGAGCCAAGGGAGCCC-3′	5′-CCGCATGCGAATTCGAGCTCTCC-3′
	Mutagenesis Forward	Mutagenesis Reverse
A374G	5′-TGCTGTTCAAGGACTGCAACCTGGAGCGCT-3′	5′-TAGCGCTCCAGGTTGCAGTCCTTGAACAGCA-3′
G1052A	5′-GACGGCGGGGAGCTCATCTGCTATGACGGCTGCCCTC-3′	5′-GAGGGCAGCCGTCATAGCAGATGAGCTCCCCGCGCT-3′
C1313del	5′-CACGACTCTTGTCTACAAGCACTGCCGCCTTC-3′	5′-GAAGGCGGAGCCGGCAGTGCTTGTAGACAAGAGTCGTG-3′

Note.—The construct with the 5′ FLAG tag and the plasmids carrying the common Finnish mutation C₈₈₉T were prepared by use of the Chameleon Double-Stranded Site-Directed Mutagenesis Kit (Stratagene); the selection primer is located in the polylinker region of the SV-vector. The remaining mutation plasmids were prepared by use of the QuikChange Site-Directed Mutagenesis Kit (Stratagene).