Table 2.
Effect of repeated freezing cycles on TAC values
| Anti-coagulant | Number of freeze/thawing cycles | ||||
| 1 | 2 | 3 | 4 | 5 | |
| K3-EDTA | 1.05 ± 0.02 | 1.00 ± 0.02 | 1.05 ± 0.03 | 1.13 ± 0.03 | 1.22 ± 0.04 |
| Heparin | 1.05 ± 0.03 | 0.95 ± 0.02 | 1.00 ± 0.03 | 1.24 ± 0.04 | 1.32 ± 0.05 |
Ten different plasma samples from normal individuals, collected on K3-EDTA or heparin, were assayed after a number of 1–5 freeze/thawing cycles, by the automated TAC assay. Values are presented as the mean ± SEM. The target value of non-freezed plasma, collected in a K3-EDTA tube was given in Table 1