Figure 4.

Immunoprecipitation with anti-nNOS antibody of solubilized HEK-293 cells co-expressing nNOS and HF-tagged full length (Flag-A, Flag-B, Flag-D) or C-terminally truncated (Flag-trA, Flag-trB, FlagtrD) α₁-AR subtypes. For comparison, immunoprecipitation of HF-tagged receptors with anti-Flag M2 affinity resin is also shown. Monomers of each full-length and truncated subtype are indicated by arrows on the left of each blot. Blots shown are representative of at least two other experiments with similar results. IP = immunoprecipitation; IB = immunoblot.