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. 2002 Aug 16;2:17. doi: 10.1186/1471-2210-2-17

Figure 6.

Figure 6

Effect of L-NAME (500 μM) on UTP (100 μM), norepinephrine (NE, 100 μM), NGF (100 ng/ml) and EGF(100 ng/ml) induced ERK phosphorylation in PC12 cells. HFα1A-transfected PC12 cells were serum-starved for 2 hr, pretreated with L-NAME for 30 min, and exposed to agonist for 15 min before harvesting. ERK activation was determined by Western blotting with dual phospho-specific ERK antibodies as described in Methods. Although the blot shown is overexposed, similar strength of signals were seen with NE, NGF and EGF after shorter exposures.