Figure 1.

Pedigrees of families with Wolfram syndrome (DIDMOAD). A, Family WS-1 shows homozygosity for markers at the chromosome 4p16 WFS1 locus. B–D, Families WS-2–WS-4 show haplotypes for markers at chromosome 4q22-24. Recombinations in individuals V.9 and V.13 from family WS-2, individual IV.2 from family WS-3, and individual IV.1 from family WS-4 define the critical region. For individuals denoted by an asterisk, genotypes were inferred from the genotypes of existing family members, and maximum-likelihood haplotypes were deduced by use of GENEHUNTER. Blackened squares denote haplotypes and recombinants, and broken lines indicate alternative crossover events that could also explain the data.