Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Dependence of triplex formation on divalent cations To analyze the dependence of triplex formation on divalent cations, DS1 (0.5 μM) was annealed to TFO (10 μM) in binding buffer (20 mM HEPES pH 7.5, 50 mM Na-acetate, 10 mM MgCl2) and in buffer not containing Mg2+-ions (20 mM HEPES pH 7.5, 50 mM Na-acetate). In the Mg2+ free buffer, no triplex formation is detectable.
