Table 1.

Cycling protocol for HBD-1, HBD-2 and GAPDH RT-PCR

Step	Denaturation time/temperature	Annealing time/temperature	Extension time/temperature	Number of cycles
HBD-1
1	1 5 min/95°C	30 sec/55°C	30 sec/72°C	1
2	30 sec/95°C	30 sec/55°C	30 sec/72°C	28
3	30 sec/95°C	30 sec 55°C	10 min/72°C	1
HBD-2
1	1 5 min/95°C	30 sec/60°C	30 sec/72°C	1
2	30 sec/95°C	30 sec/60°C	30 sec/72°C	28
3	30 sec/95°C	30 sec/60°C	10 min/72°C	1
2 GAPDH
1	5 min/95°C	30 sec/55°C or 60°C	30 sec/72°C	1
2	30 sec/95°C	30 sec/55°C or 60°C	30 sec/72°C	28
3	30 sec/95°C	30 sec/55°C or 60°C	10 min/72°C	1

¹ Taq Polymerase added at the end of the first 5 min denaturation step. ²GAPDH RT-PCR was performed as duplex PCR in concert with either HBD-1 or HBD-2