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. 1999 Oct 14;65(5):1261–1267. doi: 10.1086/302619

Figure 3.

Figure 3

Mutational analysis of CRYGD. a, Sequence chromatograms showing the single-base-pair substitution 411G→A of CRYGD (exon 2; nucleotides 404–418) found in individuals affected with the aculeiform-cataract phenotype. b, Mutational analysis (i.e., ARMS) of CRYGD exon 2. The ARMS assay shows cosegregation of the 411G→A mutation with the aculeiform-cataract phenotype in three Swiss families (families A–C). Selective amplification of a 194-bp PCR product for the 411G→A mutation is seen in affected individuals only. The 410-bp fragment amplified with primers for the marker D1S1663 is an internal control for the PCR reaction and is present in all lanes.