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. 1999 Oct 15;65(5):1308–1320. doi: 10.1086/302623

Figure 3.

Figure 3

Identification of the Val896Met mutation in exon 27 of MyBPC. A, Partial sequence of the noncoding strand of exon 27 in an individual heterozygous for the Val896Met mutation, showing the c→t transition (g→a, coding strand) that results in the amino acid substitution of Met for Val at codon 896. B, Genotyping of the Val896Met mutation, by ASREA. Products of NlaIII digestion of the 310-bp PCR-amplification product of exon 27 were separated on a 12% polyacrylamide gel. An internal NlaIII site generated fragments of 233 and 77 bp in an unaffected individual (lane 2). The creation of an NlaIII site in an HCM-affected heterozygous individual resulted in additional, 141- and 92-bp fragments (lane 1).