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. 1999 Oct 15;65(5):1308–1320. doi: 10.1086/302623

Figure 4.

Figure 4

Identification of the cytosine deletion in codon 796 in exon 24 of MyBPC. A, Partial sequence of the noncoding strand of exon 24 of MyBPC in an unaffected individual (i) and a heterozygous HCM-affected individual (ii), showing the deletion of a g (c, coding strand) at nucleotide position 2298 (first position of codon 796), which results in a shift in reading frame. B, PCR-SSCP analysis of amplified products of exon 24 of MyBPC, separated on a 10% polyacrylamide gel containing 5% glycerol. The arrows indicate the mobility shifts caused by the Δc mutation at nucleotide 2298 in a heterozygous HCM-affected individual (lane 2). The single-stranded conformers of an unaffected individual are seen in lane 1.