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Use of allele-specific oligonucleotides for MC1R genotyping. A total of 100 ng each of PCR-amplified MC1R consensus and variant product were bound both independently (homozygote) and as an equimolar mixture (heterozygote) to nylon membranes and were hybridized with consensus or variant 15-base oligonucleotides, as indicated. Hybridization and washing of the [32P]-labeled oligonucleotides, in the presence of TMAC, differentiates each of the six MC1R variants examined in this study.
