Figure 1.

Alternative splicing of human PEG1/MEST. Arrows indicate primers used for expression studies. RT was followed by PCR amplification by use of exon specific primers. Top, Human EST sequences that matched with the first exon of the alternative isoform. Middle, Exon organization of the PEG1/MEST transcription unit that transcribes two isoforms. In the present study, RT-PCR was done either with PEG36 and PEG34 or with PEG33-PEG34, to detect isoform 2 and isoform 1, respectively. Poly T's and AflII correspond to polymorphic sites in the 3′ UTR that have been described elsewhere. Riesewijk et al. (1997) and Kobayashi et al. (1997) used primer pair R4 and R10 and primer pair HP1F and HP1R, respectively. Bottom, Primers used for RT-PCR, from mouse peripheral blood.