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. 2026 Feb 9;17:25. doi: 10.1186/s40104-025-01345-z

Table 3.

Effects of HMB on short-chain fatty acids (SCFAs) in jejunal and ileal contents of Tibetan lambs (n = 6)

Items Treatments1 SEM P-value
CON L-HMB M-HMB H-HMB
Jejunum
 Total SCFAs, mmol/L 7.22b 7.89b 8.98a 7.70b 0.184  < 0.001
 Acetate, mmol/L 5.81b 6.40b 7.16a 6.13b 0.169 0.007
 Propionate, mmol/L 0.71d 0.83c 1.14a 0.94b 0.035  < 0.001
 Butyrate, mmol/L 0.60 0.57 0.59 0.53 0.018 0.552
 Valerate, mmol/L 0.10 0.09 0.09 0.10 0.006 0.704
 Acetate/Propionate 8.26a 7.82ab 6.33b 6.55b 0.267 0.032
 Acetate, % 80.44 80.93 79.51 79.49 0.482 0.774
 Propionate, % 9.84b 10.63ab 12.87a 12.29a 0.393 0.031
 Butyrate, % 8.30 7.27 6.55 6.84 0.266 0.082
 Valerate, % 1.43 1.17 1.07 1.38 0.090 0.332
Ileum
 Total SCFAs, mmol/L 12.82b 13.94b 16.04a 14.03b 0.318  < 0.001
 Acetate, mmol/L 9.83b 11.04b 12.66a 11.07b 0.285  < 0.001
 Propionate, mmol/L 1.49b 1.51b 1.89a 1.47b 0.043  < 0.001
 Butyrate, mmol/L 1.36 1.26 1.36 1.38 0.020 0.12
 Valerate, mmol/L 0.13 0.13 0.13 0.12 0.001 0.17
 Acetate/Propionate 6.58 7.32 6.72 7.59 0.170 0.10
 Acetate, % 76.63b 79.05a 78.85a 78.86a 0.360 0.04
 Propionate, % 11.70 10.94 11.81 10.46 0.222 0.089
 Butyrate, % 10.65a 9.09bc 8.52c 9.81ab 0.217  < 0.001
 Valerate, % 1.01a 0.91b 0.82c 0.87bc 0.020  < 0.001

SCFAs Short chain fatty acids

1HMB was supplemented at 0 mg/kg (CON group), 430 mg/kg (L-HMB), 715 mg/kg (M-HMB), and 1,000 mg/kg (H-HMB) of diet

a−dDifferent letters represent significant differences (P < 0.05)