Fig 2.

Histopathology of right lower leg granuloma biopsy demonstrating inflammation confined to subcutaneous fat with intralobular inflammation and mild septal thickening. (A, original magnification 4×) Granulomatous and dense lymphocytic infiltrate without lymphocytic rimming (B, 10× original magnification) and neutrophilic predominance in deep dermis (C, 20× original magnification). D, Immunohistochemical staining for rubella virus capsid antigen (red) demonstrates strong staining localizing to neutrophils (green; myeloperoxidase and blue); DAPI (4′,6-diamidino-2-phenylindole) highlighting nuclei. Monoclonal antibodies used included anti–rubella virus capsid (Centers for Disease Control and Prevention, Division of Viral Diseases), antimyeloperoxidase, and anti–CD206 antibodies.