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. 2005 Nov 23;115(12):3613–3622. doi: 10.1172/JCI25342

Figure 4.

Figure 4

Identification of TFF1 by MALDI-TOF MS and ESI-Q-TOF MS/MS. (A and B) MALDI mass spectrometric data obtained from subfractions X1 and X2. (C) Tandem mass spectra obtained from ESI-Q-TOF MS/MS were significantly matched with the peptide AQTETCTVA of human 1pS2 (gi|2392506) or TFF1. The fragment ions [y(2), y(3), etc.] generated from MS/MS analyses were numbered according to their position from the C terminus. (D) Using the MASCOT search engine, peptide masses obtained from all subfractions (X1, X2, X1′, and X2′) were significantly matched with 1pS2, the recombinant human TFF1, and the identified residues obtained from MS/MS were labeled.