Table 4.

Reactome analysis of the coding-gene module “MEgreen’ in placentas obtained from mothers affected by obesity.

System	Pathway	Significant genes	P-value	FDR
Gene Expression	PRC2 Methylates histones and DNA; RNA Polymerase I Promoter Opening; Regulation of the Endogenous Retroelements by the HUSH complex; RNA Polymerase I Promoter Escape; DNA Methylation; Regulation of Endogenous Retro elements by piRNAs; Transcription regulation by small RNAs	DNMT3A, MBD4, MTA3, RSF1, SMUG1, CDS1, KDM6, SMARCE1 H1-2, H2AC6-7-11, H2BC4- 5-6-8-12-21,H3C4-12, H4C5-8-9-14-15	0.0000002.7	0.00026
DNA Repair	Cleavage of the damaged Pyrimidine; Recognition and Association with DNA Glycosylase containing affected Purine; Cleavage of damage Purine; Depurination; Recognition and Association of DNA Glycosylase with site containing an affected Pyrimidine; Depyrimidination; Base Excision Repair;	H2AC6-7-11, H2BC4-5-6- 12, H1-2	0.0000003.6	0.00026
Disease	Defective Pyrotosis	H2BC4-5-6-12, H1-2	0.000002.2E	0.0012
Chromatin Organization	HDACs (histone deacetylate)	H2AC6-7-11, H2BC4-5-6- 12, H1-2 H4C5-8-9	0.00001	0.0025
Cell Cycle	Nucleosome Assembly; deposition of new CENPA containing nucleosome at the centromere; Packaging of Telomere Ends; G2/M DNA Damage Checkpoint	H2AC7, H2BC4-5-6, H4C8- 9-14-15	0.00001.6E	0.0031
Developmental Biology	Chromatin Modifications during the Maternal to Zygotic Transition (MZT)	H2AC7, H2BC5-6-8-12- 216KDM6A	0.0001.33	0.0182
DNA Replication	Assembly of the ORC Complex at the origin of Replication;	H2BC12-21, KPNB1	0.0001.5	0.019

Using Reactome, an open source, peer-reviewed pathway database, we analyzed genes contained in the MEgreen module negatively correlated with cbFFA in the WGCNA analysis. The p-value and FDR reported belong to the pathway with the most significant p-value listed in that specific system. The pathways reported are all the pathways with a p-value <0.05 in the Reactome analysis for all the genes listed in MEgreen.