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. 2005 Nov 18;33(20):6547–6554. doi: 10.1093/nar/gki960

Figure 2.

Figure 2

In vitro splicing of pBK-bclx in HeLa S100 extract complemented with SR proteins. pBK-bclx pre-mRNAs were spliced in in vitro splicing reactions containing HeLa S100 extract (lanes 2–8) or HeLa nuclear extract (lane 1). Equal amounts of each purified SR protein were added where indicated. Single SR proteins were normalized in activity so that they had equivalent function in activation of splicing of a substrate containing the first intron of the human β-globin gene when added to a S100 extract (data not shown). Splicing ratios and error bars have been obtained from three independent experiments.