Table 1.
Results from model selections
Ratio | Transformants | Recombinants | Positives |
---|---|---|---|
0 | 298 000 | 45 | 0/16 |
10−5 | 310 000 | 51 | 4/16 |
10−4 | 305 000 | 275 | 10/16 |
10−3 | 308 000 | 2540 | 16/16 |
Ratio, ratio of I-CreI-N75 encoding plasmid to I-DmoI encoding plasmid. Transformants, number of colonies obtained after transformation. Recombinants, number of colonies obtained after plating of 107 transformed cells on selection plates. Positive, number of clones positive by screening against C1234.