Table 3.
Results from selections of I-CreI variants by phage display
| Target | C1234 | C1221 | C4334 | S1234 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Round | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 |
| Binders (%) | 60 | 98 | 99 | 71 | 99 | 100 | 5 | 46 | 90 | 1 | 1 | 1 |
| Mutants | QRN 8 | QRN 13 | QRN 16 | QRN 14 | QRN 23 | QRN 17 | QQK 3 | QRR 15 | QRR 29 | QRA 1 | QRT 1 | QRR 1 |
| QRT 6 | QQK 7 | QQK 10 | QQK 4 | QQK 9 | QQK 9 | QRT 1 | QRK 2 | QRK 1 | ||||
| QQK 5 | QRT 3 | QRS 3 | QRT 4 | QRT 3 | QRT 3 | QRA 1 | QRN 1 | |||||
| QRA 2 | QRS 3 | QAK 2 | QRA 4 | QRR 1 | ||||||||
| QRR 1 | QHK 2 | QRR 2 | ||||||||||
| QRS 1 | QRK 1 | QRS 2 | ||||||||||
| QRK 1 | ||||||||||||
| QAK 1 | ||||||||||||
| QHK 1 | ||||||||||||
Binders, % of clones (out of 80) leading to positive signal by ELISA. Mutants, residues 44, 68 and 70 for clones positive against at least one of the targets by ELISA and number of times this sequence was found among 32 clones picked at random for each round and each target. The sequences of ELISA-negative clones are not shown. For S1234 sequence, see Materials and Methods.