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. 2005 Dec;16(12):5465–5479. doi: 10.1091/mbc.E05-04-0337

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Figure 7. — Mob1p, Dbf2p, and Cdc14p precipitate centromere DNA. (A) Centromere chromatin immunoprecipitation (ChIP) was conducted, as described in Materials and Methods. Myc-tagged-Mob1p, Dbf2p, Cdc14p, and Chl4p were immunoprecipitated from asynchronous cells. Dilutions of immunoprecipitated material (IP) were used as template for PCR to test for coprecipitating centromere DNA (CEN1, CEN3). tot, total cell extract used as positive control for PCR template. (B) ChIP of Myc-tagged Mob1p, Cdc14p, and Chl4p from conditional ndc10-1 or ndc10-42 cells.