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Ipl1p transiently localizes to anaphase spindles in mob1-77 cells, but not in cdc15-2 or cdc14-1 cells. Ipl1p-GFP was analyzed in wild-type, mob1-77, cdc15-2, and cdc14-1 cells after release from NDZ-induced metaphase arrest, as described in the text. On release from metaphase block (t = 0), the percentage of cells that displayed Ipl1p-GFP on the anaphase spindle was scored over time. The presented data are from a representative experiment.
