Figure 11.

ORP1L overexpression disturbs endocytic membrane trafficking. TRITC-dextran was internalized for 10 min into HeLa cells transfected with EGFP or EGFP-ORP1L expression plasmids, followed by chase periods of up to 120 min. The cells were fixed and immunostained with anti-Lamp-1 mAb and viewed with a confocal microscope. (A–D) EGFP-expressing control cells fixed directly after the 10-min dextran uptake. (E–H) EGFP-ORP1L-expressing cells fixed directly after the 10-min uptake period. (I–L) EGFP-expressing control cells fixed after 120-min chase. (M–P) EGFP-ORP1L-expressing cells fixed after 120-min chase. D, H, L, and P are overlays illustrating the colocalization of the internalized TRITC-dextran with Lamp-1.