Figure 5.

ORP1L interacts with Rab7-GTP in a COS cell two-hybrid assay. ORP1L in pM2 was transfected into COS cells together with wild-type Rab7, Rab7 Q67L, or Rab7 T22N (identified below the diagram) in pVP16, or the empty vector as a control (pVP16). The cells also received the pG5luc luciferase reporter plasmid and a β-galactosidase expression plasmid for normalization of values. (A) Mean luciferase activities (±SEM) of two experiments carried out in triplicate. (B) Similar expression levels of the activation domain fusions of wt Rab7 and the Q67L and T22N mutants. Equal amounts of cell lysate were Western blotted with anti-Rab7.