Figure 8.

The PHD of ORP1L interacts with phosphoinositides. GST-ORP1L PHD fusion protein (gray bars) bound to glutathione-Sepharose beads was incubated with vesicles consisting of unlabeled PC, [¹⁴C]PC, and 1 mol% of the indicated phosphoinositide. “PC” indicates binding to liposomes that consist of PC only. GST was used as a negative control (values subtracted from the results), and the PHD of PLCδ fused to GST (black bars) was used as a positive control. The lipid binding is expressed as a percentage of total radioactivity recovered. The data represents a mean from two independent experiments.