Figure 3.

Identification of transcription factors binding to the NF-IL6 and CRE sites. NCI-H292 cells were incubated with 25 μM MG132 for 1 h. Nuclear extracts were prepared and DNA-protein binding activity measured by EMSA using NF-IL6 (A) and CRE (B) oligonucleotides probe. Supershift assays were performed using 2 μg of the indicated antibodies as described in Materials and Methods. Results are representative of three independent experiments.