Figure 5.

MG132 induced recruitment of C/EBPβ, C/EBPδ, and CBP to the COX-2 promoter and acetylation of histone H3 and H4 in vivo. (A) Schematic illustration of various regulatory elements on the COX-2 promoter (–397 to +7). (B–D) NCI-H292 cells were treated with 25 μM MG132 for the indicated time or 10 ng/ml TNF-α for 60 min, and then ChIP assays were performed using anti-C/EBPβ, anti-C/EBPδ (B), anti-CBP (C), anti-acetyl H3, or anti-acetyl H4 (D) antibody or without antibody (Control) to assay the precipitated COX-2 promoter regions (–397 to –119 or –109 to +7) as described in Materials and Methods. One percent of the chromatin was assayed to verify equal loading (Input). Results are representative of three independent experiments.