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Confocal microscopic observations of cytoplasmic actin patches in the rcy1Δ mutant. KKT133 (rcy1Δ) was cultured in YPDAW at 18°C or 30°C for 9 h, followed by staining with TRITC-phalloidin as described in Figure 3A. Three consecutive z-focal planes are shown. Bar, 5 μm.
