Skip to main content
. 2005 Dec;16(12):5592–5609. doi: 10.1091/mbc.E05-05-0452

Figure 6.

Figure 6.

Cytoplasmic assembly of actin patches in the cdc50Δ erg3Δ mutant is dependent on endocytosis. (A) The effect of mutations in actin-related genes on the localization of actin patches in the cdc50Δ erg3Δ mutant. cdc50Δ erg3Δ mutants carrying an additional mutation, cdc42-1 (KKT202), bni1-116-EGFP bnr1Δ (KKT223), arp2-1 (KKT210), or las17-11 (KKT206) were cultured in YPDAW at 30°C for 8 h, followed by an additional 2 h culture at 37°C. TRITC-phalloidin staining was performed as described in Figure 3A. Five z-focal planes obtained by confocal microscopic observation are shown. Bar, 5 μm. (B) Effects of LAT-A on the localization of actin patch components in the cdc50Δ erg3Δ mutant. The strain KKT237 (PGAL1-3HA-CDC50 erg3Δ) doubly expressing Abp1p-mRFP1 and Las17p-EGFP was cultured in SDAW at 30°C for 8 h, followed by an additional 1-h culture in the presence (LAT-A) or absence (DMSO) of 100 μM LAT-A. Central confocal images are shown. Bar, 5 μm.