Table 1.
S. cerevisiae strains used in this study
| Straina | Genotype | Reference or source |
|---|---|---|
| ABY944 | MATa lys2-801 ura3-52 his3Δ-200 trp1-1 leu2-3,112 tpm1-2::LEU2 tpm2Δ::HIS3 | Pruyne et al. (1998) |
| ANS2-3A | MATα sec2-56 ura3-52 leu2-3,112 his3/4 | A gift from A. Nakano |
| ANS4-8A | MATα sec4-2 ura3-52 leu2-3,112 his3/4 | A gift from A. Nakano |
| DDY1960 | MATa lys2-801 ura3-52 his3Δ-200 leu2-3,112 ade2-101 las17-11::LEU2 | A gift from D. Drubin |
| DDY0546 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 sla2-Δ1::URA3 | A gift from D. Drubin |
| DJYD2-16D | MATα gal2 ura3 his4 trp1 leu2 cdc42-1 | Johnson and Pringle (1990) |
| RH2607 | MATa his3 his4 leu2 bar1 lcb1-100 | A gift from H. Riezman |
| YMW211U | MATa lys2-801 ura3-52 his3Δ-200 trp1-Δ63 leu2-1 ade2-101 arp2-1::URA3 | Madania et al. (1999) |
| Y3656 | MATα lys2Δ0 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 | A gift from C. Boone |
| YEF473 | MATa/α lys2-801/lys2-801 ura3-52/ura3-52 his3Δ-200/his3Δ-200 trp1Δ-63/trp1Δ-63 leu2Δ-1/leu2Δ-1 | Longtine et al. (1998) |
| BY4743 | MATa/α LYS2/lys2Δ0 ura3Δ0/ura3Δ0 his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 met15Δ0/MET15 | Brachmann CB et al. (1998) |
| KKT2 | MATa lys2Δ0 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 | This study |
| KKT7 | MATα HphMX4::PGAL1-3HA-CDC50 can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 | This study |
| KKT9 | MATα cdc50Δ::HphMX4 | This study |
| KKT12 | MATa erg3Δ::KanMX6 | This study |
| KKT16 | MATa HphMX4::PGAL1-3HA-CDC50 erg3Δ::KanMX6 | This study |
| KKT31 | MATα cdc50Δ::HphMX4 ade2Δ::HphMX4 ade3Δ::KanMX6 | This study |
| KKT32 | MATa cdc50Δ::HphMX4 ade2Δ::HphMX4 ade3Δ::KanMX6 | This study |
| KKT40 | MATa drs2Δ::KanMX6 | This study |
| KKT66 | MATα BNI1-EGFP::HIS3MX6 | This study |
| KKT67 | MATα HphMX4::PGAL1-3HA-CDC50 BNI1-EGFP::HIS3MX6 | This study |
| KKT68 | MATa erg3Δ::KanMX6 BNI1-EGFP::HIS3MX6 | This study |
| KKT69 | MATa HphMX4::PGAL1-3HA-CDC50 erg3Δ::KanMX6 BNI1-EGFP::HIS3MX6 | This study |
| KKT90 | MATa HphMX4::PGAL1-3HA-CDC50 erg3Δ::KanMX6 MYO5-EGFP::HIS3MX6 | This study |
| KKT108 | MATa LAS17-EGFP::HIS3MX6 | This study |
| KKT109 | MATa HphMX4::PGAL1-3HA-CDC50 LAS17-EGFP::HIS3MX6 | This study |
| KKT110 | MATα erg3Δ::KanMX6 LAS17-EGFP::HIS3MX6 | This study |
| KKT111 | MATα HphMX4::PGAL1-3HA-CDC50 erg3Δ::KanMX6 LAS17-EGFP::HIS3MX6 | This study |
| KKT121 | MATa GIC1-EGFP::HIS3MX6 | This study |
| KKT122 | MATa HphMX4::PGAL1-3HA-CDC50 GIC1-EGFP::HIS3MX6 | This study |
| KKT123 | MATα erg3Δ::KanMX6 GIC1-EGFP::HIS3MX6 | This study |
| KKT124 | MATa HphMX4::PGAL1-3HA-CDC50 erg3Δ::KanMX6 GIC1-EGFP::HIS3MX6 | This study |
| KKT141 | MATα lem3Δ::HphMX4 | This study |
| KKT158 | MATα HphMX4::PGAL1-3HA-CDC50 erg3Δ::KanMX6 ABP1-EGFP::HIS3MX6 | This study |
| KKT163 | MATα HphMX4::PGAL1-3HA-CDC50 erg3Δ::KanMX6 PAN1-EGFP::HIS3MX6 | This study |
| KKT190 | MATα drs2Δ::HIS3MX6 | This study |
| KKT202 | MATa HphMX4::PGAL1-3HA-CDC50 erg3Δ::KanMX6 cdc42-1 | This study |
| KKT206 | MATα HphMX4::PGAL1-3HA-CDC50 erg3Δ::KanMX6 las17-11::LEU2 | This study |
| KKT210 | MATa HphMX4::PGAL1-3HA-CDC50 erg3Δ::KanMX6 arp2-1::URA3 | This study |
| KKT219 | MATα HphMX4::PGAL1-3HA-CDC50 erg3Δ::KanMX6 SLA2-EGFP::HIS3MX6 | This study |
| KKT223 | MATa CaURA3::PGAL1-3HA-CDC50 erg3Δ::KlLEU2 bnil-116-EGFP::KanMX6 bnr1Δ::HphMX4 | This study |
| KKT229 | MATa MYO5-EGFP::KanMX6 ABP1-mRFP1::HIS3MX6 | This study |
| KKT232 | MATa CaURA3::PGAL1-3HA-CDC50 erg3Δ::KlLEU2 MYO5-EGFP::KanMX6 ABP1-mRFP1::HIS3MX6 | This study |
| KKT234 | MATα LAS17-EGFP::KanMX6 ABP1-mRFP1::HIS3MX6 | This study |
| KKT237 | MATα CaURA3::PGAL1-3HA-CDC50 erg3Δ::KlLEU2 LAS17-EGFP::KanMX6 ABP1-mRFP1::HIS3MX6 | This study |
| KKT246 | MATa myo3Δ::HIS3MX6 myo5-1::KanMX6 | This study |
| KKT247 | MATα cdc50Δ::HphMX4 ade2Δ::HphMX4 ade3Δ::KanMX6 erg3 p[CDC50, URA3, ADE3] | This study |
| KKT248 | MATa lcb1-100 | This study |
| KKT251 | MATα erg3Δ::KlLEU2 | This study |
| KKT252 | MATa erg4Δ::KanMX6 | This study |
| KKT254 | MATa sec2-56 | This study |
| KKT256 | MATa sec4-2 | This study |
| YKT477b | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 tpm1-2::LEU2 tpm2Δ::HIS3 | This study |
| YKT532b | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 bnil-116-EGFP::KanMX6 bnr1Δ::hphMX4 | Kadota et al. (2004) |
a
KKT strains are isogenic derivatives of BY4743. For KKT strains, only relevant genotypes are described.
b
YKT477 and 532 strains are isogenic derivatives of YEF473.