Figure 9.

UHRF1 mRNA (A) and protein (B) is down-regulated in response to genotoxic and cytotoxic agents in A549 cells. A549 cells were treated with different agents and then 24 h after initiation of treatment, cells were harvested for either quantitative PCR analysis of UHRF1 mRNA or Western blot analysis of UHRF1 protein and the loading control, actin. Data for PCR analysis are shown as a mean ± SD of triplicate reactions. Cells were treated with 100 μM etoposide or 40 μM cis-platinum for 3 h at 37°C and washed with PBS, and then fresh medium was added. Other drugs were left in the medium until cells were harvested (20 nM taxol, 1 mM hydroxyurea, 330 nM nocodazole, and 3 mU/ml bleomycin).