Figure 1.

Schematic diagram of ChIP cloning (SACHI) procedure. The technology consists of five steps (A–E). Details are given in Materials and Methods. Briefly, chromatin is cross-linked (A) and immunoprecipitated (B) to enrich for chromatin fragments containing RE1 binding sites. DNA is then purified and polished, and adaptors are ligated (C). RE1 containing fragments are further enriched by nested PCR (D) before subcloning and analysis (E).