Figure 5.

Validation of the isolated RE1 sites from ChIP cloning using in vivo ChIP assay. Thirty-three RE1 containing regions were tested in conventional ChIP for REST occupancy in ES cells (black bars), NS cells (gray bars), and hippocampus (white bars). Only the RE1 associated with Cspg4 showed no enrichment for REST over background in any cell types, demonstrating the high efficiency of SAHI to identify bona fide REST targets.