Figure 2.
The G1 cCEN requires microtubules for kinetochore assembly. Cells expressing Dam1-myc9 were arrested in G1 with αFin galactose and then treated with (+NOC) or without (–NOC) the microtubule-depolymerizing drug nocodazole. Subsequently, the cultures were split into media containing GAL or GLU while maintaining the nocodazole treatment in αF. ChIP was performed with anti-myc antibody. Relative units indicate the cCEN:PGK1 ratio and the amount of Dam1-myc9 in GLU-NOC is normalized to a value of 1.0. Dam1 localization to the cCEN in G1 depends on microtubules.