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. 2005 Dec;16(12):5699–5709. doi: 10.1091/mbc.E05-03-0204

Figure 8.

Figure 8.

REP15 depletion by siRNA specifically reduces B-Tfn recycling from the ERC. (A) HeLa cells stably expressing cMycREP15 were transfected with REP15 or control (Con) siRNA duplexes for 72 h and then examined by Western analysis as indicated. Untransfected (Un) cells stably expressing cMycREP15 were used as an additional control. (B) HeLa cells were transfected with control (Con) or REP15 siRNAs and used to measure B-Tfn recycling from the ERC using a B-Tfn ELISA. All values are the mean of triplicate values and are expressed as Rfu per microgram of protein. Statistical differences between experimental and control sets were observed (*p < 0.01; ANOVA). (C) Control and REP15 siRNAs were transfected into HeLa cells as described in B, and B-Tfn recycling from the SEs was measured using a B-Tfn ELISA. No difference in B-Tfn recycling from the SEs was detected after REP15 depletion.