Table 1.
REP15 interacts with GTP-bound Rab15 but not Rab5 or Rab11. β-Galactosidase activity in Rfu was determined in yeast diploid strains coexpressing the indicated bait constructs in pLexA and prey constructs in pB42AD
| pLexA Construct | pB42AD Construct | Rfu | Interaction |
|---|---|---|---|
| Rab15 WT | REP15 (3-236) | 121,884.6 ± 6679.9 | + |
| Rab15 Q67L | REP15 (3-236) | 113,834.0 ± 6361.9 | + |
| Rab15 T22N | REP15 (3-236) | 8629.2 ± 180.7 | – |
| Rab15 N1211 | REP15 (3-236) | 17,743.8 ± 2410.8 | – |
| Rab15 Q67L | REP15 (3-151) | 20,699.7 ± 342.9 | – |
| Rab15 Q67L | REP15 (151-236) | 12,720.6 ± 164.4 | – |
| Rab15 Q67L | REP15 (100-236) | ND | – |
| Rab5 WT | REP15 (3-236) | 4072.0 ± 623.7 | – |
| Rab5 Q79L | REP15 (3-236) | 3224.0 ± 1015.2 | – |
| Rab5 S34N | REP15 (3-236) | 501.7 ± 500.2 | – |
| Rab5 N1331 | REP15 (3-236) | 9503.7 ± 1333.2 | – |
| Rab11 Q70L | REP15 (3-236) | 26,407.3 ± 1193.1 | – |
| Rab11 S25N | REP15 (3-236) | 1825.3 ± 563.6 | – |
ND, not determined.
Values represent the means ± SE of three independent studies. Statistically significant interactions (+) between proteins are indicated (p < 0.001; analysis of variance [ANOVA]).