Table 2.
Changes in expression level of selected markers of dedifferentiation and multi-lineage differentiation potential in NCCIT extract-treated cells
| NCCIT extract-treated cells (fold up-regulation)a
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Name | GenBank accession no. | Description | NCCIT cells (fold up-regulation)a | Wk 1 | Wk 2 | Wk 4 | Wk 8 | |||||||
| Somatic cell markers | ||||||||||||||
| LMNA | M13452.1 | Nuclear lamin A | 0.0015 | 0.9 | 0.0011 | 0.0014 | 0.0011 | |||||||
| LMNB1 | NM_005573.1 | Nuclear lamin B1 | 2.5 | 1.7 | 1.0 | 1.5 | 1.4 | |||||||
| LMNB2 | M94363 | Nuclear lamin B2 | 0.8 | 1.1 | 1.1 | 0.9 | 1.1 | |||||||
| NPR3 | NM_000908.1 | Atrionatriuretic peptide receptor C | 0.001 | 0.001 | 0.001 | 0.001 | 0.001 | |||||||
| Embryonic, germ cell and stem cell markers | ||||||||||||||
| OCT4 (POU5F1) and Oct4-responsive genes | ||||||||||||||
| POU5F1 | NM_002701.1 | POU domain, class 5, TF1 (Oct4) | 36 | 2 | 17 | 35 | 36 | |||||||
| UTF1 | N_003577.1 | Undifferentiated embryonic cell transcription factor 1 | 317 | 2 | 213 | 293 | 318 | |||||||
| REX1 | AJ243797 | Deoxyribonuclease III (Drn3) | 5 | 1 | 2.5 | 6 | 9 | |||||||
| FOXD3 | N_012183.1 | Forkhead box D3 (FoxD3) | 847 | 38 | 283 | 295 | 254 | |||||||
| Telomerase and telomerase-associated factors | ||||||||||||||
| TERT | N_003219.1 | Telomerase reverse transcriptase | 102 | 2.5 | 22 | 76 | 89 | |||||||
| TERF1 | N_017489.1 | Telomerase-associated factor 1 | 3.5 | 3 | 3.2 | 4.5 | 3.5 | |||||||
| TERF2 | N_005652.1 | Telomerase-associated factor 2 | 0.7 | 0.5 | 0.9 | 0.6 | 0.6 | |||||||
| SOX2 | AI356682 | Sex determining region Y-box 2 (Sox2) | 119 | 1 | 12 | 118 | 121 | |||||||
| POU3F1 | L26494.1 | POU domain, class 3, TF1 (Oct-6) | 164 | 1.5 | 3 | 106 | 84 | |||||||
| ALP1 | M13077 | Placental alkaline phosphatase 1 | 1311 | 38 | 292 | 928 | 927 | |||||||
| ALP1 | J04948.1 | Placental alkaline phosphatase 1 | 2124 | 56 | 344 | 651 | 1337 | |||||||
| CD44 | N_00610.1 | CD44 antigen (homing function) | 1028 | 102 | 205 | 622 | 663 | |||||||
| LIF | N_002309.2 | Leukemia inhibitory factor | 221 | 7 | 27 | 127 | 271 | |||||||
| FZD9 | N_003508.1 | Frizzled (Drosophila) homologue 9 | 331 | 10 | 101 | 102 | 107 | |||||||
| TEF | U06935.1 | Thyrotroph embryonic factor | 423 | 69 | 126 | 222 | 273 | |||||||
| SCGF | D86586.1 | Stem cell growth factor β | 934 | 234 | 917 | 939 | 906 | |||||||
| GCNF | AF004291.1 | Germ cell nuclear factor | 1105 | 27 | 71 | 424 | 696 | |||||||
| SPINK2 | N_021114.1 | Serine protease inhibitor, Kazal type, 2 | 662 | 2.5 | 65 | 257 | 555 | |||||||
| DKK2 | N_014421.1 | Dickkopf (Xenopus laevis) homolog 2b | 1122 | 345 | 457 | 473 | 476 | |||||||
| INTA6 | AV733308 | Integrin α6 | 3.1 | 1.5 | 4.5 | 10 | 15 | |||||||
| Markers of potential lineage- specific differentiation | ||||||||||||||
| Osteogenic lineage | ||||||||||||||
| BMP1 | N_006129.2 | Bone morphogenic protein 1 | 870 | 67 | 169 | 367 | 470 | |||||||
| BMP2 | NM_001200.1 | Bone morphogenic protein 2 | 655 | 166 | 333 | 653 | 663 | |||||||
| OGN | N_014057.1 | Osteoglycin | 17 | 2 | 4 | 14 | 17 | |||||||
| CTSK | N_000396.1 | Cathepsin K | 8 | 4 | 5 | 5 | 6 | |||||||
| TNFRSF11B | N_002546.1 | Osteoprotegerin | 82 | 1 | 14 | 94 | 65 | |||||||
| Endothelial lineage | ||||||||||||||
| VWF | N_000552.2 | Von Willebrand Factor | 629 | 604 | 598 | 734 | 647 | |||||||
| NOS3 | N_000603.1 | Nitric oxide synthase 3 (endothelial cell) | 421 | 127 | 167 | 129 | 306 | |||||||
| Myogenic lineage | ||||||||||||||
| MYF5 | N_005593.1 | Myogenic factor 5 | 13 | 10 | 13 | 30 | 23 | |||||||
| TMP1 | N_000366.1 | Tropomyosin 1 alpha | 46 | 14 | 12 | 11 | 16 | |||||||
| MYH11 | N_022844.1 | Myosin, heavy polypeptide 11 | 98 | 4 | 22 | 28 | 69 | |||||||
| Neurogenic lineage | ||||||||||||||
| NTS | N_006183.2 | Neurotensin | 73 | 7 | 20 | 27 | 48 | |||||||
| NRG1 | N_004495.1 | Neuregulin 1 isoform gamma | 857 | 60 | 56 | 66 | 86 | |||||||
| MBP | M13577.1 | Myelin basic protein | 21 | 2 | 7 | 11 | 14 | |||||||
| MOBP | D28114.1 | Myelin-associated oligodend. basic protein | 43 | 22 | 24 | 24 | 22 | |||||||
| NCAM1 | BF348061 | Neural cell adhesion molecule 1 | 18 | 1 | 7 | 17 | 20 | |||||||
| CD56 | U63041.1 | Neural cell adhesion molecule CD56 | 16 | 1.5 | 2 | 4 | 11 | |||||||
| Adipogenic lineage | ||||||||||||||
| APOA2 | N_001643.1 | Apolipoprotein A-II | 13 | 4 | 5 | 9 | 9 | |||||||
| APOD | N_001647.1 | Apolipoprotein D | 11 | 3 | 11 | 10 | 12 | |||||||
| APOE | N33009 | Apolipoprotein E | 21 | 2 | 11 | 22 | 2 | |||||||
| APOC1 | N_001645.2 | Apolipoprotein C1 | 4 | 0.5 | 1.5 | 3 | 4 | |||||||
| PPARG2 | N_015869.1 | Peroxisome prolif. activated receptor γ2 | 80 | 1 | 1.5 | 10 | 22 | |||||||
| FAD1 | BG165833 | Fatty acid desaturase 1 | 10 | 3 | 4 | 7 | 5 | |||||||
| Chondrogenic lineage | ||||||||||||||
| COL4A3 | U02520 | Collagen type IV alpha 3 | 36 | 3 | 9 | 27 | 31 | |||||||
| COL5A2 | N_000393.1 | Collagen, type V, alpha 2 | 44 | 2 | 4 | 12 | 16 | |||||||
| COL8A1 | BE877796 | Collagen, type VIII, alpha 1 | 19 | 1 | 5 | 9 | 15 | |||||||
| COL11A1 | J04177 | Collagen, type X1, alpha 1 | 251 | 1 | 10 | 42 | 230 | |||||||
| CSPG2 | N_004385.1 | Chondroitin sulfate proteoglycan 2 | 85 | 1 | 10 | 92 | 87 | |||||||
| AGC1 | N_013227.1 | Aggrecan 1 | 15 | 5.5 | 4.5 | 50 | 46 | |||||||
| DSPG3 | N_004950.2 | Dermatan sulphate proteoglycan 3 | 310 | 1.5 | 3 | 52 | 157 | |||||||
| CSPG1 | X17406.1 | Aggrecan 1 | 9 | 1 | 2 | 13 | 16 | |||||||
| FNP | X02761.1 | Fibronectin precursor | 9 | 4 | 9 | 4 | 2 | |||||||
| FN1 | AK026737.1 | Fibronectin 1 | 12 | 4 | 10 | 7 | 6 | |||||||
a
Relative to 293T cells
b
Also up-regulated in cells exposed to 293T or Jurkat extract