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. 2005 Dec;16(12):5819–5831. doi: 10.1091/mbc.E05-07-0685

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Copyright © 2005, The American Society for Cell Biology

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Figure 2. — UPR elicits polyribosome breakdown and accumulation of 80S monosomes in the cytosolic and ER fractions. Cytosol (A and B), total membrane-derived (C and D) and rough microsome-derived (E and F) fractions from control (A, C, and E) and 10 mM DTT-treated (B, D, and F) J558L cells were sedimented through 15-50% linear sucrose gradients for 3 h at 151,000 × g. Ribosomes were monitored by UV absorbance (260 nm) with the downward facing arrow denoting the 80S monosome peak. Total RNA isolated from individual gradient fractions was resolved on denaturing agarose gels. Immunoblots were performed from individual gradient fractions for translocon markers Sec61α and TRAPα.