Figure 7.

ER-bound ribosomes preferentially participate in mRNA translation during the UPR-NIH 3T3 cells. Cytosol (A and B) and total membrane-derived ER (C and D) fractions from control (A and C) and DTT-treated (B and D) NIH 3T3 cells were sedimented through 15-50% linear sucrose gradients for 3 h at 151,000 × g. Ribosomes were monitored by UV absorbance (260 nm), and the downward facing arrow denotes the 80S monosome. RNA was isolated from individual gradient fractions and used for Northern blot analysis as indicated.