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. 2005 Dec;16(12):5843–5856. doi: 10.1091/mbc.E05-07-0629

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Copyright © 2005, The American Society for Cell Biology

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Figure 9. — Atg9 trafficking to the PAS is impaired in the act1-159 mutant. (A) Atg9 distribution in act1-159 cells is normal. Wild-type (FRY245) and act1-159 (IRA004) strains expressing Atg9-RFP were grown at semipermissive temperature to midlog phase in SMD medium or incubated in SD-N for an additional 1 h and then imaged. (B) Atg9 fails to reach the PAS in the atg1Δ deletion background. The atg1Δ (IRA006) and act1-159 atg1Δ (IRA005) strains carrying a genomic Atg9-RFP fusion were grown and imaged as in A. DIC, differential interference contrast.