Table 2.
Actin mutant phenotypes
| Mutant | Cvt pathway (24°C) | Mitochondrial morphology (24°C) | Cvt pathway (37°C) | Mitochondrial morphology (37°C) | Actin dynamic defect |
|---|---|---|---|---|---|
| Wild type | + | + | + | + | None |
| act1-101 (D363A, E364A) | + | + | – | – | Cable formation, delocalized patches |
| act1-102 (K359A, E361A) | + | + | + | + | None |
| act1-105 (E311A, R312A) | + | + | + | + | ? |
| act1-111 (D222A, E224A, E226A) | + | + | – | – | Cable formation, delocalized patches |
| act1-121 (E83A, K84A) | +/- | + | – | + | Delocalized patches |
| act1-122 (D80A, D81A) | + | + | + | + | ? |
| act1-133 (D24A, D25A) | – | – | – | – | Cable formation |
| act1-135 (E4A) | + | + | + | + | None |
| act1-157 (D157E) | +/- | ND | ND | ND | None |
| act1-159 (V159N) | – | + | – | + | Disassembly |
Precursor Ape1 transport was determined with pulse-chase radiolabeling experiments followed by immunoprecipitation, whereas the mitochondrial reticulum structure was analyzed by staining cells with Mitofluor Red. The plus sign indicates the complete processing of prApe1 or normal mitochondrial morphology. The minus sign describes the aberrant organization of this organelle or prApe1 accumulation ≥ 70%. The plus/minus sign specifies that a 50% block of prApe1 transport was observed. The mutant phenotypes with regard to actin dynamics that have been reported in the literature are also indicated (Wertman et al., 1992; Drubin et al., 1993; Smith et al., 1995; Belmont and Drubin, 1998; Wolven et al., 2000). The strains that were further analyzed are highlighted in bold. ND, not determined.